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rabbit anti human antibody asic3 (Cell Signaling Technology Inc)

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Cell Signaling Technology Inc rabbit anti human antibody asic3

Effects of hypercapnic acidosis on the expression of <t>ASIC3</t> in A549 cells. A. Representative images of ASIC3 expression in different groups of A549 cells (immunofluorescence staining). The green fluorescence shows ASIC3, the blue fluorescence shows the nucleus, and the combined pink shows the expression of ASIC3 in A549 cells. B. ASIC3 fluorescence intensity results were showed in each group. The data are shown as the mean ± sd. ** P < .01, n = 5for each experiment. C. RT-qPCR analysis of the expression levels of ASIC3.The data are shown as the mean ± sd. ** P < .01, n = 3 denotes independent biological replicates from separate cell cultures.

Rabbit Anti Human Antibody Asic3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+anti+human+antibody+asic3/pmc13010014-63-1-8?v=Cell+Signaling+Technology+Inc
Average 86 stars, based on 1 article reviews

rabbit anti human antibody asic3 - by Bioz Stars, 2026-07

86/100 stars

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1) Product Images from "High ASIC3 Expression Correlates with Poor Prognosis in Lung Cancer Patients and Mediates Hypercapnic Acidosis-Induced EMT in A549 Cells"

Article Title: High ASIC3 Expression Correlates with Poor Prognosis in Lung Cancer Patients and Mediates Hypercapnic Acidosis-Induced EMT in A549 Cells

Journal: Technology in Cancer Research & Treatment

doi: 10.1177/15330338261434666

Effects of hypercapnic acidosis on the expression of ASIC3 in A549 cells. A. Representative images of ASIC3 expression in different groups of A549 cells (immunofluorescence staining). The green fluorescence shows ASIC3, the blue fluorescence shows the nucleus, and the combined pink shows the expression of ASIC3 in A549 cells. B. ASIC3 fluorescence intensity results were showed in each group. The data are shown as the mean ± sd. ** P < .01, n = 5for each experiment. C. RT-qPCR analysis of the expression levels of ASIC3.The data are shown as the mean ± sd. ** P < .01, n = 3 denotes independent biological replicates from separate cell cultures.

Figure Legend Snippet: Effects of hypercapnic acidosis on the expression of ASIC3 in A549 cells. A. Representative images of ASIC3 expression in different groups of A549 cells (immunofluorescence staining). The green fluorescence shows ASIC3, the blue fluorescence shows the nucleus, and the combined pink shows the expression of ASIC3 in A549 cells. B. ASIC3 fluorescence intensity results were showed in each group. The data are shown as the mean ± sd. ** P < .01, n = 5for each experiment. C. RT-qPCR analysis of the expression levels of ASIC3.The data are shown as the mean ± sd. ** P < .01, n = 3 denotes independent biological replicates from separate cell cultures.

Techniques Used: Expressing, Immunofluorescence, Staining, Fluorescence, Quantitative RT-PCR

HCA induces an epithelial-to-mesenchymal transition of A549 cells. A. A549 cells were incubated with HCA or amiloride (block ASIC3) and stained with the indicated antibodies of E-cadherin. Images were visualized under a microscope. Magnification, 20×. Scale bar, 20 μm. B. Statistical analysis of E-cadherin protein expressions was performed as above. C. A549 cells were stimulated withHCA or amiloride for 5 days, and cell lysates were subjected to western blot analysis with the indicated antibodies of E-cadherin and Vimentin. E-cadherin (D.) and Vimentin (E.) protein relative expressions. The data are shown as the mean ± sd. ** P < .01. n = 3 denotes independent biological replicates from separate cell cultures.

Figure Legend Snippet: HCA induces an epithelial-to-mesenchymal transition of A549 cells. A. A549 cells were incubated with HCA or amiloride (block ASIC3) and stained with the indicated antibodies of E-cadherin. Images were visualized under a microscope. Magnification, 20×. Scale bar, 20 μm. B. Statistical analysis of E-cadherin protein expressions was performed as above. C. A549 cells were stimulated withHCA or amiloride for 5 days, and cell lysates were subjected to western blot analysis with the indicated antibodies of E-cadherin and Vimentin. E-cadherin (D.) and Vimentin (E.) protein relative expressions. The data are shown as the mean ± sd. ** P < .01. n = 3 denotes independent biological replicates from separate cell cultures.

Techniques Used: Incubation, Blocking Assay, Staining, Microscopy, Western Blot

siASIC3 inhibits HCA-induced EMT in A549 cells. A. Successful silencing of ASIC3 and downregulation of ASIC3 mRNA were achieved. B. The downregulation of ASIC3 mRNA was verified by RT-PCR. C. The expression levels of the epithelial marker E-cadherin, and mesenchymal markers N-cadherin, fibronectin, and vimentin were detected. D. Quantitative analysis of the relative protein expression levels was performed. The data are shown as the mean ± sd. ** P < .01. n = 3 denotes independent biological replicates from separate cell cultures.

Figure Legend Snippet: siASIC3 inhibits HCA-induced EMT in A549 cells. A. Successful silencing of ASIC3 and downregulation of ASIC3 mRNA were achieved. B. The downregulation of ASIC3 mRNA was verified by RT-PCR. C. The expression levels of the epithelial marker E-cadherin, and mesenchymal markers N-cadherin, fibronectin, and vimentin were detected. D. Quantitative analysis of the relative protein expression levels was performed. The data are shown as the mean ± sd. ** P < .01. n = 3 denotes independent biological replicates from separate cell cultures.

Techniques Used: Reverse Transcription Polymerase Chain Reaction, Expressing, Marker

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Journal: Technology in Cancer Research & Treatment

Article Title: High ASIC3 Expression Correlates with Poor Prognosis in Lung Cancer Patients and Mediates Hypercapnic Acidosis-Induced EMT in A549 Cells

doi: 10.1177/15330338261434666

Figure Lengend Snippet: Effects of hypercapnic acidosis on the expression of ASIC3 in A549 cells. A. Representative images of ASIC3 expression in different groups of A549 cells (immunofluorescence staining). The green fluorescence shows ASIC3, the blue fluorescence shows the nucleus, and the combined pink shows the expression of ASIC3 in A549 cells. B. ASIC3 fluorescence intensity results were showed in each group. The data are shown as the mean ± sd. ** P < .01, n = 5for each experiment. C. RT-qPCR analysis of the expression levels of ASIC3.The data are shown as the mean ± sd. ** P < .01, n = 3 denotes independent biological replicates from separate cell cultures.

Article Snippet: The rabbit anti-human antibody ASIC3 was purchased from Cell Signaling Technology Inc., and anti-rabbit secondary antibodies were purchased from KangChen Bio-tech.

Techniques: Expressing, Immunofluorescence, Staining, Fluorescence, Quantitative RT-PCR

Journal: Technology in Cancer Research & Treatment

Article Title: High ASIC3 Expression Correlates with Poor Prognosis in Lung Cancer Patients and Mediates Hypercapnic Acidosis-Induced EMT in A549 Cells

doi: 10.1177/15330338261434666

Figure Lengend Snippet: HCA induces an epithelial-to-mesenchymal transition of A549 cells. A. A549 cells were incubated with HCA or amiloride (block ASIC3) and stained with the indicated antibodies of E-cadherin. Images were visualized under a microscope. Magnification, 20×. Scale bar, 20 μm. B. Statistical analysis of E-cadherin protein expressions was performed as above. C. A549 cells were stimulated withHCA or amiloride for 5 days, and cell lysates were subjected to western blot analysis with the indicated antibodies of E-cadherin and Vimentin. E-cadherin (D.) and Vimentin (E.) protein relative expressions. The data are shown as the mean ± sd. ** P < .01. n = 3 denotes independent biological replicates from separate cell cultures.

Article Snippet: The rabbit anti-human antibody ASIC3 was purchased from Cell Signaling Technology Inc., and anti-rabbit secondary antibodies were purchased from KangChen Bio-tech.

Techniques: Incubation, Blocking Assay, Staining, Microscopy, Western Blot

Journal: Technology in Cancer Research & Treatment

Article Title: High ASIC3 Expression Correlates with Poor Prognosis in Lung Cancer Patients and Mediates Hypercapnic Acidosis-Induced EMT in A549 Cells

doi: 10.1177/15330338261434666

Figure Lengend Snippet: siASIC3 inhibits HCA-induced EMT in A549 cells. A. Successful silencing of ASIC3 and downregulation of ASIC3 mRNA were achieved. B. The downregulation of ASIC3 mRNA was verified by RT-PCR. C. The expression levels of the epithelial marker E-cadherin, and mesenchymal markers N-cadherin, fibronectin, and vimentin were detected. D. Quantitative analysis of the relative protein expression levels was performed. The data are shown as the mean ± sd. ** P < .01. n = 3 denotes independent biological replicates from separate cell cultures.

Article Snippet: The rabbit anti-human antibody ASIC3 was purchased from Cell Signaling Technology Inc., and anti-rabbit secondary antibodies were purchased from KangChen Bio-tech.

Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing, Marker

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1) Product Images from "High ASIC3 Expression Correlates with Poor Prognosis in Lung Cancer Patients and Mediates Hypercapnic Acidosis-Induced EMT in A549 Cells"

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